Gene Editing Analysis: Now Faster than Ever
INDUCE-seq® is a scalable, PCR-free platform for rapidly mapping both on-target and off-target DNA breaks induced during genome editing. Delivering data in as little as 2 days from sample processing, this cell-based assay offers single-nucleotide resolution, capturing each DNA break with a single sequencing read. Designed for high sensitivity, reproducibility, and genome-wide, unbiased detection, INDUCE-seq® is compatible with any gene editing system and adaptable to all cell types, including primary cells, iPSCs, and immortalized cells from diverse tissues.
- On- and off- target assessment
- Editing kinetic analysis
- Guide screening
- Nuclease development
- Editing strategy optimizations
The INDUCE-seq® On-Demand Solution
INDUCE-seq®, rapid, high-resolution detection of double-stranded breaks (DSBs) is now available as an in-lab solution, giving developers maximum control over timelines and experimental design. INDUCE-seq® On-Demand empowers developers to perform library preparation and sequencing in their own labs, then gain access to 51¸£Àû analysis pipeline to customize data reports.
Why CGT Developers Choose INDUCE-seq®
INDUCE-seq® delivers the industry’s most comprehensive and accurate on- and off-target characterization service, enabling pharmaceutical companies to make confident go/no-go decisions early in discovery while minimizing regulatory risk throughout development.
- Comprehensive detection: Quantifies on/off target DSBs across the genome
- Cost Savings: Reduce R&D timelines and risk of regulatory holds due to off-target effects
- Cell-based assay: Compatible with all relevant cell types, including primary cells, iPSCs, T-cells, and immortalized cells from diverse tissues
- Single-nucleotide resolution: Precise DSB detection at the single nucleotide level.
- PCR-free workflow: Directly measure DSBs without amplification bias, 1 read = 1 break.
- Fast turnaround: Sample to insights in days.
- Universal compatibility: Technology is compatible with any gene editing system.
- Automation capability: Integrate with industry-standard automation systems.
INDUCE-seq® is also available through a full partnership service model. The INDUCE-seq® services offer the same accuracy and efficiencies in identifying on- and off-target gene editing, while allowing for higher levels of customization of analysis parameters. Learn more about INDUCE-seq® services here.