Breakome Profiling for Damage and Disease Insights
INDUCE-seq庐 is a highly sensitive, PCR-free sequencing method that maps DNA double-strand breaks (DSBs) genome-wide at single-nucleotide resolution. Originally developed to evaluate off-target effects of gene editing, INDUCE-seq also enables comprehensive profiling of the “breakome”: the full landscape of DSBs present in a cell population at a given time. This includes breaks caused by gene editing, endogenous cellular processes, disease pathology, and environmental exposures. Its broad detection capability makes INDUCE-seq a powerful tool for research and therapeutic development across diverse biological and clinical contexts.
- Unbiased, genome-wide detection of DSBs
- Breakome signatures as disease biomarkers
- Discovery stage lead candidate selection
- Detection of on- and off- target gene editing events
- Comparative assessment of healthy and diseased tissue
INDUCE-seq庐 Technology
INDUCE-seq provides a window into the dynamic and disease-relevant breakome landscape. As research into disease progression moves increasingly toward molecular-level, mechanistic insights, INDUCE-seq offers a unique and actionable tool to detect and interpret genomic damage with precision.
- PCR Free
Ensures linear representation of break frequency without amplification bias. - High Sensitivity
Detects rare or low-frequency breaks that might be missed by other techniques. - Single-Nucleotide Resolution
Enables precise localization of breaks, aiding in mechanistic interpretation and biomarker discovery.
By capturing and quantifying endogenous, random, and induced DSBs in a single method, INDUCE-seq enables researchers to distinguish background DNA damage from editing events and assess overall genome integrity.
INDUCE-seq庐 Offering
INDUCE-seq庐, rapid, high-resolution detection of double-stranded breaks (DSBs) is now available as an in-lab solution, giving developers maximum control over timelines and experimental design, as well as in the traditional full-service model.
INDUCE-seq庐 On- Demand
INDUCE-seq庐 Services
- In-lab solution: Control timelines and experimental designs by running INDUCE-seq assay in your own lab.
- Fast turnaround: Enables high-throughput, rapid turnaround to uncover double stranded breaks in days not months.
- Collaborative Analysis: Clients can access proprietary analysis platform to customize visualizations and reports.
- Outsourced solution: Send your samples to Broken String, where experts perform the assay and data analysis.
- Comprehensive reporting: Receive detailed insights including on/off-target effects, break distribution, and genome-wide profiling.
- Scientific consultation: Includes expert guidance and support for data interpretation and experimental planning.